Enterococcus faecalis Tissier | Type strain | DSM 20478, ATCC 19433, NCDO 581, NCIB 775, NCTC 775, WDCM 00009, CCUG 19916, JCM 8726

Strain identifier

BacDive ID: 5292

Type strain:

Species: Enterococcus faecalis

Strain Designation: Tissier

Culture col. no.: DSM 20478, ATCC 19433, NCDO 581, NCIB 775, NCTC 775, WDCM 00009, CCUG 19916, JCM 8726

Strain history: <- NCDO <- NIRD; Tissier

Section

Name and taxonomic classification
Morphology
Culture and growth conditions
Physiology and metabolism
Safety information
Sequence information

External links

Enterococcus faecalis Tissier is a microaerophile, mesophilic bacterium of the family Enterococcaceae.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Lactobacillales
	Family	Enterococcaceae
	Genus	Enterococcus
	Species	Enterococcus faecalis
	Full Scientific Name (PNU)	Enterococcus faecalis (Andrewes and Horder 1906) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecalis

Information on morphological and physiological propertiesMorphology

[Ref.: #8847]

Incubation period

1-2 days

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8847]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), microaerophilic

[Ref.: #8847]

Culture medium growth

[Ref.: #8847]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #8847]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), microaerophilic

[Ref.: #8847]

Culture medium growth

[Ref.: #8847]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #36538]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #36538]

Culture medium growth

[Ref.: #36538]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8847]			growth	37 ̊C
[Ref.: #36538]			growth	37 ̊C

[Ref.: #8847]	Temperature range	mesophilic
[Ref.: #36538]	Temperature range	mesophilic

Information on physiology and metabolismPhysiology and metabolism

[Ref.: #8847]

Oxygen tolerance

microaerophile

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Reference

[Ref.: #8847]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
24
12
30
26
30-32
16
16-18
26
resistant0
30
30
26-28
16
12-14
20
24-26
10
18
18-20
resistant0
resistant0
22-24
22
resistant0
14
12-14
30
20
10-12
26
24
30
resistant0
22
20
28

[Ref.: #8847]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22
10
30
24-26
32
16
16-18
26
resistant0
26
30-32
24-26
16
12-14
20
26
10
18
18
resistant0
resistant0
22-24
20-22
resistant0
14
14
30
20
14
22
26
30
resistant0
22
20
28

[Ref.: #8847]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22
8-10
30-32
24-26
30-32
18
16-18
20-22
resistant0
30
32-34
26
14
10
20
26
8-10
18
16-18
resistant0
resistant0
26
18-20
resistant0
14
12
32-34
20-22
12
26-28
22-24
30
resistant0
22
20
28

[Ref.: #8847]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
24-26
12
30
26
30-32
18
18-20
28
resistant0
30
32
26-28
14
12
22
26
10
18
18-20
resistant0
resistant0
26
20
resistant0
14
14
32-34
22
12
26
26
30
resistant0
24
20
30

[Ref.: #8847]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20-22
10
30
26
30
16
16
24
resistant0
28-30
28-30
26
12-14
10-12
20
24
10
18
16
resistant0
resistant0
20
20
resistant0
12
12
30
20
12
26
22
28
resistant0
22
18
24-26

[Ref.: #8847]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
20-22
10
30
26
30
16
16
24
resistant0
28-30
28-30
26
12-14
10-12
20
24
10
18
16
resistant0
resistant0
20
20
resistant0
12
12
30
20
12
26
22
28
resistant0
22
18
24-26

Only first 15 entries are displayed. Click here to see all.

[Ref.: #22913]

Name of produced compound

Acetoin

[Ref.: #8847]	Murein short key	A11.06
[Ref.: #8847]	Murein types	A3alpha L-Lys-L-Ala₃

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22915]	2-dehydro-D-gluconate ChEBI	+/-	builds acid from
[Ref.: #22915]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #22915]	amygdalin ChEBI	+	builds acid from
[Ref.: #22915]	arabinose ChEBI	-	builds acid from
[Ref.: #22915]	arbutin ChEBI	+	builds acid from
[Ref.: #22913]	arginine ChEBI	+	hydrolysis
[Ref.: #22915]	cellobiose ChEBI	+	builds acid from
[Ref.: #22915]	citrate ChEBI	+	growth
[Ref.: #22915]	D-arabinose ChEBI	-	builds acid from
[Ref.: #22915]	D-arabitol ChEBI	-	builds acid from
[Ref.: #22915]	D-fructose ChEBI	+	builds acid from
[Ref.: #22915]	D-fucose ChEBI	-	builds acid from
[Ref.: #22915]	D-glucose ChEBI	+	builds acid from
[Ref.: #22915]	D-lyxose ChEBI	-	builds acid from
[Ref.: #22915]	D-mannose ChEBI	+	builds acid from
[Ref.: #22915]	D-tagatose ChEBI	+	builds acid from
[Ref.: #22915]	D-xylose ChEBI	-	builds acid from
[Ref.: #22915]	erythritol ChEBI	-	builds acid from
[Ref.: #22915]	galactitol ChEBI	-	builds acid from
[Ref.: #22915]	galactose ChEBI	+	builds acid from
[Ref.: #22915]	gentiobiose ChEBI	+	builds acid from
[Ref.: #22913]	gluconate ChEBI	+	builds acid from
[Ref.: #22915]	gluconate ChEBI	+/-	builds acid from
[Ref.: #22915]	glycerol ChEBI	+	builds acid from
[Ref.: #22915]	glycogen ChEBI	-	builds acid from
[Ref.: #22913]	hippurate ChEBI	-	hydrolysis
[Ref.: #22914]	hippurate ChEBI	+	hydrolysis
[Ref.: #22915]	inulin ChEBI	-	builds acid from
[Ref.: #22914]	L-arabinose ChEBI	-	builds acid from
[Ref.: #22915]	L-arabinose ChEBI	-	builds acid from
[Ref.: #22915]	L-arabitol ChEBI	-	builds acid from
[Ref.: #22915]	L-fucose ChEBI	-	builds acid from
[Ref.: #22915]	L-sorbose ChEBI	-	builds acid from
[Ref.: #22913]	L-xylose ChEBI	-	builds acid from
[Ref.: #22915]	L-xylose ChEBI	-	builds acid from
[Ref.: #22914]	lactose ChEBI	+	builds acid from
[Ref.: #22915]	lactose ChEBI	+	builds acid from
[Ref.: #22915]	malate ChEBI	+	growth
[Ref.: #22915]	maltose ChEBI	+	builds acid from
[Ref.: #22913]	mannitol ChEBI	+	builds acid from
[Ref.: #22914]	mannitol ChEBI	+	builds acid from
[Ref.: #22915]	mannitol ChEBI	+	builds acid from
[Ref.: #22914]	melezitose ChEBI	+	builds acid from
[Ref.: #22915]	melezitose ChEBI	+/-	builds acid from
[Ref.: #22915]	melibiose ChEBI	-	builds acid from
[Ref.: #22913]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #22915]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #22915]	methyl alpha-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #22913]	methyl D-glucoside ChEBI	-	builds acid from
[Ref.: #22915]	methyl D-glucoside ChEBI	-	builds acid from
[Ref.: #22915]	myo-inositol ChEBI	-	builds acid from
[Ref.: #22915]	N-acetyl-D-glucosamine ChEBI	+	builds acid from
[Ref.: #22915]	pyruvate ChEBI	+	growth
[Ref.: #22915]	raffinose ChEBI	-	builds acid from
[Ref.: #22915]	rhamnose ChEBI	+/-	builds acid from
[Ref.: #22915]	ribitol ChEBI	-	builds acid from
[Ref.: #22915]	ribose ChEBI	+	builds acid from
[Ref.: #22915]	salicin ChEBI	+	builds acid from
[Ref.: #22915]	serine ChEBI	+	growth
[Ref.: #22913]	sorbitol ChEBI	+	builds acid from
[Ref.: #22915]	sorbitol ChEBI	+/-	builds acid from
[Ref.: #22913]	sucrose ChEBI	+	builds acid from
[Ref.: #22915]	sucrose ChEBI	+	builds acid from
[Ref.: #22914]	tagatose ChEBI	+	builds acid from
[Ref.: #22915]	tagatose ChEBI	+	builds acid from
[Ref.: #22915]	trehalose ChEBI	+	builds acid from
[Ref.: #22915]	turanose ChEBI	-	builds acid from
[Ref.: #22914]	urea ChEBI	-	hydrolysis
[Ref.: #22915]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #22913]		acetoin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #22913]		acetoin ChEBI	+

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #8847]		catalase	-	1.11.1.6
[Ref.: #8847]		cytochrome-c oxidase	-	1.9.3.1

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #47075]	744	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8847]	7957	+	+	+	-	-	+	+	+	-	+	+	-	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7919	+	+	+	-	-	+	+	+	+/-	+	+	-	+	-	-	-	+	-	-	+	+	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7877	+	+	+	-	-	+	+	+	+	+	+	-	+	-	-	+	+	-	+/-	+	+	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7583	+	+	+	-	-	+	+	+	-	+	+	-	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7582	+	+	+	-	-	-	+	+	-	+	+	-	+	-	-	+	+	-	-	-	-	-	+	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7581	+	+	-	-	-	-	+	+	-	+	+	-	+	-	-	+	+	-	-	+	-	+	-	-	-	+	-	-	+	+	+/-	-
[Ref.: #8847]	7580	+	+	-	-	-	-	+	+	-	+	+	-	+	-	-	+	+	-	-	+	-	+	-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7579	+	+	-	-	-	-	+	+	-	+	+	-	+	-	-	-	+	-	-	+	-	+	-	-	-	+	-	-	+	+	+	+
[Ref.: #8847]	7578	+	+	-	-	-	-	+	+	-	+	+	-	+	-	-	+	+	-	-	+	-	+	+/-	-	-	+	-	-	+	+	+	-
[Ref.: #8847]	7577	+	+	+	-	-	+	+	+	-	+	+	-	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	-	+	+	+	-

Information on possible application of the strain and its possible interaction with e.g. potential hostsSafety information

[Ref.: #8847]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassSequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Enterococcus faecalis strain ATCC 19433 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY351321	226	ENA	1351 tax ID	*
[Ref.: #20218]	Enterococcus faecalis gene for 16S ribosomal RNA, partial sequence	D31675	199	ENA	1351 tax ID	*
[Ref.: #20218]	Enterococcus faecalis strain ATCC 19433 16S ribosomal RNA gene, partial sequence	DQ411814	1483	ENA	1351 tax ID	*
[Ref.: #20218]	Enterococcus faecalis strain DSM 20478 16S ribosomal RNA gene, partial sequence	HQ012029	936	ENA	1351 tax ID	*
[Ref.: #20218]	Enterococcus faecalis (NCTC 775) 16S ribosomal RNA, 3' end, 23S ribosomal RNA, 5' end, and transfer RNA-Ala gene	L16515	418	ENA	1351 tax ID	*
[Ref.: #20218]	E.faecalis 16S-23S rRNA spacer DNA	X87182	226	ENA	1351 tax ID	*
[Ref.: #20218]	E.faecalis 16S rRNA gene, partial (strain ATCC 19433 (CCUG 19916))	Y12905	366	ENA	1351 tax ID	*
[Ref.: #8847]	Enterococcus faecalis gene for 16S rRNA, partial sequence	AB012212	1517	ENA	1351 tax ID
[Ref.: #8847]	Enterococcus faecalis partial 16S rRNA gene, type strain DSM 20478T	LN681572	1513	ENA	1351 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus faecalis ATCC 19433	GCA_000392875	scaffold	GenBank	1169286 tax ID	*
[Ref.: #66792]	Enterococcus faecalis NCTC775	GCA_900447895	contig	GenBank	1351 tax ID	*
[Ref.: #66792]	Enterococcus faecalis ATCC 19433	1169286.3	wgs	PATRIC	1169286 tax ID	*
[Ref.: #66792]	Enterococcus faecalis strain NCTC775	1351.1230	wgs	PATRIC	1351 tax ID	*

[Ref.: #22915]

GC-content

38.6 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collectionsExternal links

[Ref.: #8847]

Culture collection no.

DSM 20478, ATCC 19433, NCDO 581, NCIB 775, NCTC 775, WDCM 00009, CCUG 19916, JCM 8726

[Ref.: #20218]

Associated Passport(s) in StrainInfo

52284, 52281, 100149, 133736, 52288, 866270

References

#8847

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20478

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22913

R. Maarit Niemi, Tuula Ollinkangas, Lars Paulin, Pavel Svec, Peter Vandamme, Antti Karkman, Marcel Kosina, Kristina Lindström: Enterococcus rivorum sp. nov., from water of pristine brooks. IJSEM 62: 2169 - 2173 2012 ( DOI 10.1099/ijs.0.038257-0 , PubMed 22058322 )

#22914

Viridiana Sistek, Andrée F. Maheux, Maurice Boissinot, Kathryn A. Bernard, Philippe Cantin, Ilse Cleenwerck, Paul De Vos, Michel G. Bergeron: Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. IJSEM 62: 1314 - 1320 2012 ( DOI 10.1099/ijs.0.029033-0 , PubMed 21788227 )

#22915

Karl H. Schleifer, Renate Kilpper-Bälz: Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.. IJSEM 34: 31 - 34 1984 ( DOI 10.1099/00207713-34-1-31 )

#36538 ; Curators of the CIP;
#47075 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 19916

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These References are textmined

Change proposal

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